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by: null.In vivo and in vitro fluorescence spectral imaging of intracellular pH (pHi) in Dictyostelium discoideum.
Recent studies in our laboratory have used fluorescence spectral imaging to quantify intracellular pH (pHi) during Dictyostelium development. Cells expressing a fluorescent protein (GFP) targeted to the mitochondria are used to follow changes in pHi. The relationship of fluorescence intensity to intracellular pH was quantified using in vivo and in vitro measurements. During development, the pHi increases as cells develop, reaching a maximum at the end of the generation time. Intracellular acidification of the developing cells was found to be due to the loss of extracellular K+ and the uptake of HCO3- from the medium. In vitro measurements at the end of the normal generation time were found to be comparable to the in vivo measurements, confirming that the fluorescence intensity changes reflect intracellular pH changes. The ratio of fluorescence intensity from 510 to 420 nm (I510/I420) was sensitive to small changes in pHi of the cellular organelles. The I510/I420 ratio can be used as a useful parameter to investigate the effect of various metabolic, developmental or other intracellular perturbations on pH.Q:
Get a list of all parent nodes while recursing
I’m trying to achieve the following recursive parent node search that would print all of its children starting from the original parent node (root) downwards. I don’t want to count total number of parent nodes because I’ll just have to traverse it again but rather want to print out a list of all parent nodes and how many are their children.
There’s quite a bit of related posts but none of them quite cover all cases. For example, here’s one that uses a variable to count the total but doesn’t mention printing the parent nodes for nested cases.
Given the following structure (just using a simple tree to explain my problem):
Grocery items -> Cereals -> Cornflakes -> Salmon
Suppose I start from https://blankbookingagency.com/?p=239326
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Cognitive And Emotional Responses To Life Narratives Edition by Barry Blaustein and Publisher Academic Press. Save up to 80% by choosing the eTextbook option for ISBN: 9781442280348, 1442280339. The print version of this textbook is ISBN: 9780230202488, 0230202490.Attention is paid to the application of an ink containing an organic solvent to an ink jet recording method in recent years. However, since organic solvents have an influence on the environment, it is desirable to conduct recording with an ink not containing an organic solvent. As a method for conducting recording with an ink not containing an organic solvent, an ink containing a water-soluble organic solvent and water is known
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